Syllabus:
1. Compare structure and organisation of both pro- and eukaryotic chromosomes, in terms of size, packing of DNA, linear/ circular, presence of introns and type of regulator sequences.
2. Describe structure and function of eukaryotic DNA that code for neither protein nor RNA, with reference to intron, centromere, telomere, promoter, enhancer and silencer. (Regulation @ Transcriptional level)
3. Describe process and significance of gene amplification, eg. rRNA.
4. Describe eukaryotic processing of pre-mRNA: intron splicing, polyadenylation and 5' capping.
5. Define 'control elements' and explain how control elements & other factors (TF, repressors, histone modification & DNA methylation) influences transcription.
6. State how gene expression is controlled at translational and post-translational level.
7. Outline differences between prokaryotic and eukaryotic control of gene expression.
The reason why I listed the syllabus objectives is to show you that there are some things that you can save brain power on. For example, you only need to state the ways of gene expression regulation at the translation and post-translational level.
1. Compare structure and organisation of both pro- and eukaryotic chromosomes, in terms of size, packing of DNA, linear/ circular, presence of introns and type of regulator sequences.
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| TFs: Transcription factors; TIC: Transcription Initiation Complex |
2. Describe structure and function of eukaryotic DNA that code for neither protein nor RNA, with reference to intron, centromere, telomere, promoter, enhancer and silencer. (Regulation @ Transcriptional level)
Useful information. Keep sharing your expertise!
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